Designing siRNA/Chitosan-Methacrylate Complex Nanolipogel for Prolonged Gene Silencing Effects

Despite the great therapeutics potential, sustained release of small interfering RNA (siRNA) remains a challenge. Development siRNA nanoparticle provides possibility to further enhance therapeutic efficacy in gene-based therapy. Herein, we present new system based on encapsulation siRNA/chitosan-methacrylate (CMA) complexes into liposomes form UV crosslinkable Nanolipogels (NLGs) with siRNA-release properties, both vitro and inside cells. We demonstrated that CMA nanogel NLGs can efficiency provide up 28 days. To understand mechanism cellular uptake, multiple endocytic inhibitors have been used study endocytosis pathways. This proved positively charged enter cells via pathways, facilitating endosome escape slowly releasing cytoplasm. Transfection experiments confirmed crosslinked NLG delivery effective transfection prolonged silencing effect for 14 expect this sustained-release platform would be interest fundamental biological studies clinical applications extend use siRNA-based therapies.